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hand2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology hand2
    Hand2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hand2+antibody/dHAND+Antibody/pm41707997-181-63-64
    Average 93 stars, based on 18 article reviews
    hand2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Regulation of De Novo Ceramide Synthesis: The Role of Dihydroceramide Desaturase and Transcriptional Factors NFATC and Hand2 in the Hypoxic Mouse Heart
    Article Snippet: Revelation was carried out using a horseradish peroxidase coupled secondary antibody and the Western Blot ECL kit from GE (RPN 2232). .. Fifty micrograms of whole cell extracts from AD293 cells overexpressing Hand2 was immunoprecipitated with the Hand2 antibody (sc-22818X; Santa Cruz biotechnologies) in the presence of either the same amount of proteins from mock transfected AD293 cells, or cells transfected with NFATC1. .. The reactions were incubated in the presence of Protein G using the MagnaBead system from Invitrogen.

    Article Title: Regulation of De Novo Ceramide Synthesis: The Role of Dihydroceramide Desaturase and Transcriptional Factors NFATC and Hand2 in the Hypoxic Mouse Heart
    Article Snippet: Revelation was carried out using a horseradish peroxidase coupled secondary antibody and the Western Blot ECL kit from GE (RPN 2232). .. Coimmunoprecipitation Fifty micrograms of whole cell extracts from AD293 cells overexpressing Hand2 was immunoprecipitated with the Hand2 antibody (sc-22818X; Santa Cruz biotechnologies) in the presence of either the same amount of proteins from mock transfected AD293 cells, or cells transfected with NFATC1. .. The reactions were incubated in the presence of Protein G using the MagnaBead system from Invitrogen.

    Transfection:

    Article Title: Regulation of De Novo Ceramide Synthesis: The Role of Dihydroceramide Desaturase and Transcriptional Factors NFATC and Hand2 in the Hypoxic Mouse Heart
    Article Snippet: Revelation was carried out using a horseradish peroxidase coupled secondary antibody and the Western Blot ECL kit from GE (RPN 2232). .. Fifty micrograms of whole cell extracts from AD293 cells overexpressing Hand2 was immunoprecipitated with the Hand2 antibody (sc-22818X; Santa Cruz biotechnologies) in the presence of either the same amount of proteins from mock transfected AD293 cells, or cells transfected with NFATC1. .. The reactions were incubated in the presence of Protein G using the MagnaBead system from Invitrogen.

    Article Title: Regulation of De Novo Ceramide Synthesis: The Role of Dihydroceramide Desaturase and Transcriptional Factors NFATC and Hand2 in the Hypoxic Mouse Heart
    Article Snippet: Revelation was carried out using a horseradish peroxidase coupled secondary antibody and the Western Blot ECL kit from GE (RPN 2232). .. Coimmunoprecipitation Fifty micrograms of whole cell extracts from AD293 cells overexpressing Hand2 was immunoprecipitated with the Hand2 antibody (sc-22818X; Santa Cruz biotechnologies) in the presence of either the same amount of proteins from mock transfected AD293 cells, or cells transfected with NFATC1. .. The reactions were incubated in the presence of Protein G using the MagnaBead system from Invitrogen.

    Incubation:

    Article Title: Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma
    Article Snippet: Tumor tissue sections were de-paraffinized and antigen retrieval was achieved with sodium citrate (0.01 M, pH6) for antigen retrieval, followed by quenching in 0.3% H 2 O 2 for 20 min and permeabilization in 0.05% Saponin (Fluka) for 5 min. .. The sections were blocked by incubating with 2% BSA 1× PBS supplemented with 1% horse serum at room temperature and incubated with HAND2 antibody (Santa Cruz Biotechnology A-12, Sc-398167, at dilution 1:100) overnight at 4 °C. .. Samples were than washed with 1× PBS and incubated with the secondary universal antibody (Vectastain, Vector laboratories Inc., Burlingame, CA, USA, PK-6200), for 45 min at room temperature, according to manufacturer instructions.

    other:

    Article Title: Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice
    Article Snippet: Immunostaining Immunohistochemistry was performed in 5 -μ m-thick, 10% neutral buffer formalin-fixed paraffin-embedded sections using anti-active Rac1 (1 : 500, NewEast Biosciences, Malvern, PA, USA), Rac1 (1 : 500; BD Biosciences, San Jose, CA, USA), P450SCC (1 : 100, Santa Cruz, Dallas, TX, USA), 3 β -HSD (1 : 100, Santa Cruz), Muc1 (1 : 200, Abcam, Cambridge, UK), FKBP52 (1 : 100, Abcam), Hand2 (1 : 200, Santa Cruz), ER α (1 : 100, Dako, Glostrup, Denmark) and PR (1 : 100, Dako), Ki67 (1 : 500, Epitomics, Burlingame, CA, USA), Cox2 (1 : 500, Santa Cruz), TNF α (1 : 500, Bioworld, St. Louis Park, MN, USA), P38 (1 : 100, Cell Signaling Technology, Danvers, MA, USA), p-P38 (1 : 100, Cell Signaling Technology) and cleaved Caspase-3 (1 : 100, Cell Signaling Technology) antibodies, respectively.

    Article Title: Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.
    Article Snippet: To determine the quantitative expression of HAND2 in MPM and non-tumoral specimens, obtained cDNA was used in SYBR-Green PCR assay and reactions were run on 7500 Fast Real-time PCR system thermal cyclers (Applied Biosystems).

    Article Title: Characterization of Molecular Changes in Endometrium Associated With Chronic Use of Progesterone Receptor Modulators: Ulipristal Acetate Versus Mifepristone
    Article Snippet: Fluoromount-G with 4′, 6-diamidino-2-phenylindole (DAPI) was purchased from eBiosciences, Hatfield, PA. Endometrial sections or endometrial stromal cells were incubated with 1 or more of the following primary antibodies: HAND2 (1:250, Santa Cruz Biotechnology, Santa cruz, CA; SC-9409), FGF18 (1:100, Santa Cruz Biotechnology, Santa Cruz, CA; SC-393471), PR (1:100, DAKO, Denmark; DAKO-A0098), and Progesterone receptor B isoform (PRB) 22 (1:300, Cell Signaling, Beverly, MA; CST-31575).



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    QC6352 impacts the chromatin accessibility of ADRN CRC TFs in MYCN -amplified cells (A and B) Adrenergic or mesenchymal median score in response to DMSO or 100 nM of QC6352 treatment for 48 h in adrenergic dominant cell lines (BE2C, SIMA, and KELLY) (A) and mesenchymal dominant cell lines (SK-N-AS and SK-N-SH) (B). KDM4 inhibition results in enhanced MES scoring with reduced ADRN signatures in all tested cell lines (red arrow indicates direction of signature change). (C) HOMER Motif analysis showing the predicted adrenergic CRC TF binding motifs in which genome-wide chromatin accessibility is impacted by QC6352 treatment of SIMA cells. (D) PHOX2A, ASCL1, and MYCN motif densities around ATAC-seq open chromatin regions (±1,000 bp) by categories in SIMA cells. Motif density was determined by the HOMER program and normalized to that in a background sequence of equal length. (E) STRING network analysis of transcriptional factors predicted to bind the regions with reduced chromatin accessibility by QC6352 treatment of SIMA cells. The expression threshold for genes encoding TFs is set as log2CPM > 1. CPM, counts per million mapped reads. Red color indicates ADRN CRC TFs. Blue color indicates MES CRC TFs. (F) IGV snapshots show chromatin accessibility at MYCN , <t>HAND2</t> , PHOX2B , and ALK loci is reduced by QC6352 in SIMA and BE2C cells. (G) Density heatmaps for CUT&TAG of KDM4A-C genomic binding in BE2C cells, and ChIP-seq density heatmaps of the indicated histone marks in BE2C cells after 48 h treatment with control or 200 nM QC6352, ranked by MYCN read intensity within ± 5 kb of peak summits. MYCN ChIP-seq data were retrieved from GEO SuperSeries GSE94824. (H) The indicated epigenetic marks upregulated and downregulated by QC6352 treatment of BE2C cells for 48 h followed by ChIP-seq or CUT&TAG analyses. (I) The global distribution of H3K9me3, H3K36me3, H3K27Ac, H3K4me1, and H3K27me3 peaks at different regions of annotated genes. 3′ UTR, 3′ untranslated region; 5′ UTR, 5′ untranslated region; TSS, transcription start site. (J) Snapshot of using the IGV program displaying the KDM4s, H3K9me3, H3K36me3, H3K27me3, H3K27Ac, and H3K4me1 peaks at the PHOX2B and ASCL1 genomic loci.
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    Image Search Results


    QC6352 impacts the chromatin accessibility of ADRN CRC TFs in MYCN -amplified cells (A and B) Adrenergic or mesenchymal median score in response to DMSO or 100 nM of QC6352 treatment for 48 h in adrenergic dominant cell lines (BE2C, SIMA, and KELLY) (A) and mesenchymal dominant cell lines (SK-N-AS and SK-N-SH) (B). KDM4 inhibition results in enhanced MES scoring with reduced ADRN signatures in all tested cell lines (red arrow indicates direction of signature change). (C) HOMER Motif analysis showing the predicted adrenergic CRC TF binding motifs in which genome-wide chromatin accessibility is impacted by QC6352 treatment of SIMA cells. (D) PHOX2A, ASCL1, and MYCN motif densities around ATAC-seq open chromatin regions (±1,000 bp) by categories in SIMA cells. Motif density was determined by the HOMER program and normalized to that in a background sequence of equal length. (E) STRING network analysis of transcriptional factors predicted to bind the regions with reduced chromatin accessibility by QC6352 treatment of SIMA cells. The expression threshold for genes encoding TFs is set as log2CPM > 1. CPM, counts per million mapped reads. Red color indicates ADRN CRC TFs. Blue color indicates MES CRC TFs. (F) IGV snapshots show chromatin accessibility at MYCN , HAND2 , PHOX2B , and ALK loci is reduced by QC6352 in SIMA and BE2C cells. (G) Density heatmaps for CUT&TAG of KDM4A-C genomic binding in BE2C cells, and ChIP-seq density heatmaps of the indicated histone marks in BE2C cells after 48 h treatment with control or 200 nM QC6352, ranked by MYCN read intensity within ± 5 kb of peak summits. MYCN ChIP-seq data were retrieved from GEO SuperSeries GSE94824. (H) The indicated epigenetic marks upregulated and downregulated by QC6352 treatment of BE2C cells for 48 h followed by ChIP-seq or CUT&TAG analyses. (I) The global distribution of H3K9me3, H3K36me3, H3K27Ac, H3K4me1, and H3K27me3 peaks at different regions of annotated genes. 3′ UTR, 3′ untranslated region; 5′ UTR, 5′ untranslated region; TSS, transcription start site. (J) Snapshot of using the IGV program displaying the KDM4s, H3K9me3, H3K36me3, H3K27me3, H3K27Ac, and H3K4me1 peaks at the PHOX2B and ASCL1 genomic loci.

    Journal: Cell Reports Medicine

    Article Title: Histone lysine demethylase 4 family proteins maintain the transcriptional program and adrenergic cellular state of MYCN-amplified neuroblastoma

    doi: 10.1016/j.xcrm.2024.101468

    Figure Lengend Snippet: QC6352 impacts the chromatin accessibility of ADRN CRC TFs in MYCN -amplified cells (A and B) Adrenergic or mesenchymal median score in response to DMSO or 100 nM of QC6352 treatment for 48 h in adrenergic dominant cell lines (BE2C, SIMA, and KELLY) (A) and mesenchymal dominant cell lines (SK-N-AS and SK-N-SH) (B). KDM4 inhibition results in enhanced MES scoring with reduced ADRN signatures in all tested cell lines (red arrow indicates direction of signature change). (C) HOMER Motif analysis showing the predicted adrenergic CRC TF binding motifs in which genome-wide chromatin accessibility is impacted by QC6352 treatment of SIMA cells. (D) PHOX2A, ASCL1, and MYCN motif densities around ATAC-seq open chromatin regions (±1,000 bp) by categories in SIMA cells. Motif density was determined by the HOMER program and normalized to that in a background sequence of equal length. (E) STRING network analysis of transcriptional factors predicted to bind the regions with reduced chromatin accessibility by QC6352 treatment of SIMA cells. The expression threshold for genes encoding TFs is set as log2CPM > 1. CPM, counts per million mapped reads. Red color indicates ADRN CRC TFs. Blue color indicates MES CRC TFs. (F) IGV snapshots show chromatin accessibility at MYCN , HAND2 , PHOX2B , and ALK loci is reduced by QC6352 in SIMA and BE2C cells. (G) Density heatmaps for CUT&TAG of KDM4A-C genomic binding in BE2C cells, and ChIP-seq density heatmaps of the indicated histone marks in BE2C cells after 48 h treatment with control or 200 nM QC6352, ranked by MYCN read intensity within ± 5 kb of peak summits. MYCN ChIP-seq data were retrieved from GEO SuperSeries GSE94824. (H) The indicated epigenetic marks upregulated and downregulated by QC6352 treatment of BE2C cells for 48 h followed by ChIP-seq or CUT&TAG analyses. (I) The global distribution of H3K9me3, H3K36me3, H3K27Ac, H3K4me1, and H3K27me3 peaks at different regions of annotated genes. 3′ UTR, 3′ untranslated region; 5′ UTR, 5′ untranslated region; TSS, transcription start site. (J) Snapshot of using the IGV program displaying the KDM4s, H3K9me3, H3K36me3, H3K27me3, H3K27Ac, and H3K4me1 peaks at the PHOX2B and ASCL1 genomic loci.

    Article Snippet: HAND2 (dHAND) , Santa Cruz , sc-398167, NA.

    Techniques: Amplification, Inhibition, Binding Assay, Genome Wide, Sequencing, Expressing, ChIP-sequencing, Control

    Journal: Cell Reports Medicine

    Article Title: Histone lysine demethylase 4 family proteins maintain the transcriptional program and adrenergic cellular state of MYCN-amplified neuroblastoma

    doi: 10.1016/j.xcrm.2024.101468

    Figure Lengend Snippet:

    Article Snippet: HAND2 (dHAND) , Santa Cruz , sc-398167, NA.

    Techniques: Recombinant, Modification, Synthesized, Saline, Transfection, Apoptosis Assay, Purification, Transgenic Assay, Control, Expressing, Plasmid Preparation, Software